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Certifications, Licenses & Compliance

Avand Kashef Tasnim (AKT) is a legally registered company (Company Reg. No. 51850) holding official permits from the competent authorities. All operations are conducted and recorded under formal regulatory oversight, in full compliance with applicable laws and standards. Our services and product quality are approved by leading laboratories and the relevant regulatory and health authorities.

Regulatory Licenses

QA/QC & Analytical Methods

Representative methods used in venom profiling and quality control. SOP codes and method summaries are available for audit on request; deliverables include versioned PDFs (chromatograms with peak tables/totals, LC-MS(/MS) summaries, SDS-PAGE gels, and protein% reports).

Key methods & SOP references
Method SOP Code Brief Availability
LC-MS SOP-AN-LCMS-01 ESI LC-MS(/MS) peptide/protein profiling; C18 reversed-phase with water/0.1% TFA (A) & acetonitrile/0.1% TFA (B); Orbitrap-class MS (e.g., Q Exactive HF-X), Top-N DDA, full MS m/z 200–1200 (~60k). Outputs: TICs, MS/MS IDs, batch QC summaries. Samples stored at −80 °C; runs tracked with timestamped metadata. SOP & sample report available on request
HPLC SOP-AN-HPLC-02 Fractionation & purity check with PDA 210/215 nm (Knauer Smartline); C18 4.6×250 mm, 5 µm, 100 Å; A: water+0.1% TFA, B: ACN+0.1% TFA; 0.5 mL/min; 30 µL injection. Deliverables: peak tables (RT/Area/Height), totals, PDA peak-purity, and column performance metrics; retention-time consistency checked across batches. SOP & chromatogram pack available on request
SDS-PAGE SOP-AN-SDSP-03 Tricine SDS-PAGE (Coomassie R-250); ladder ticks 116–14.4 kDa. Venom typically shows predominant <10 kDa bands. Used for batch comparability and low-MW enrichment checks; gel images archived with run metadata. SOP & representative gel available on request
Protein % (Kjeldahl) SOP-AN-KJ-04 AOAC 981.10 total nitrogen → protein (factor 6.25); ~100 mg sample; H2SO4 + copper catalyst; 420 °C/2 h; neutralization & titration. Reported as batch protein% with calculation worksheet. SOP & example worksheet available on request
LD50 (mouse, IP) SOP-AN-LD50-05 Acute toxicity (research) under prior ethical approval; standardized murine IP protocol. Preference for in vitro surrogates; LD50 values disclosed only when validated in-study and approved for release. SOP & redacted summary available on request

Batch-level Certificates of Analysis (CoA) and QC packs (HPLC/PDA, LC-MS(/MS), SDS-PAGE, protein%) are available on request.

Audit & Inspection Readiness

We maintain records for batch traceability (IDs → CoAs → raw analytical data), staff competency, equipment calibration logs, and SOP version control. To request an audit pack or schedule a remote/on-site review, please contact our team.

FAQ

How often are certificates updated?

Renewal cycles depend on the issuing authority. We update the PDFs and status badges promptly after renewal.

How can I obtain a batch CoA?

Share your batch ID via the contact form; we’ll provide the CoA and method summaries under NDA if required.

Do you provide clinical-grade materials?

We supply research-grade materials. Clinical-grade supply would require additional GMP/GDP compliance, which can be discussed case-by-case.